Composite

Part:BBa_K5048017:Design

Designed by: Yecheng Zhao   Group: iGEM24_WHU-China   (2024-09-13)


pBAD-Lpp'OmpA-DDDDK-QEP-GST-dt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 239
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 74
    Illegal AgeI site found at 655
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 56
    Illegal SapI.rc site found at 837


Design Notes

We replace the T7 promoter with araBAD promoter to prevent the leakage of promoter


Source

complete synthesis

References